Publications

2018

Sviatlana Shashkova* and Adam J M Wollman,* Stefan Hohmann, Mark C Leake. Characterising Maturation of GFP and mCherry of Genomically Integrated Fusions in Saccharomyces cerevisiae. Bio-protocol, 8, 2

Victoria A. Lund, Katarzyna Wacnik, Robert D. Turner, Bryony E. Cotterell, Samuel J. Fenn, Christa G. Walther, Fabien Grein, Adam J. M. Wollman, Mark C. Leake, Nicolas Olivier, Ashley Cadby, Stephané Mesnage, Simon Jones, Simon Foster                    Molecular coordination mechanism of Staphylococcus aureus cell wall construction eLife, 7:e32057

2017

Beattie, T.R., Kapadia, N., Nicolas, E., Uphoff, S., Wollman, A.J.M., Leake, M.C. & Reyes-Lamothe, R
Synthesis by the bacterial replisome occurs during frequent exchange of the DNA polymerase
eLife, ;6:e21763 2017

Shashkova, S.* and Wollman, A.J.M.,* Leake, M.C., Hohmann, S
The yeast Mig1 transcriptional repressor is dephosphorylated by glucose-dependent and independent mechanisms
FEMS Microbiology Letters, 364,14, 2017

Wollman, A.J.M.* and Shashkova, S.,* Hedlund, E.G., Friemann, R., Hohmann, S., Leake, M.C.
Transcription factor clusters regulate genes in eukaryotic cells
eLife, 6:e274512017

Dunn, K.E., Leake, M.C., Wollman, A.J.M., Trefzer, M.A., Johnson, S.  and Tyrrell, A.M.
An experimental study of a synthetic autonomous rotary DNA nanomotor driven by strand displacement.
Open Science, 4 (3), 160767 2017

Helen Miller, Zhaokun Zhou, Jack Shepherd, Adam J M Wollman, Mark C Leake
Single-molecule techniques in biophysics: a review of the progress in methods and applications
In Press, Reports on progress in physics, 2017

2016

Adam J M Wollman and Mark C Leake
Single Molecule Narrowfield Microscopy of Protein-DNA binding dynamics in glucose signal transduction of live yeast cells
Chromosom. Archit. Methods Protoc., 5–15.2016

Adam J M Wollman, Aisha Syeda, Peter McGlynn, Mark C Leake
Single-molecule observation of DNA replication repair pathways in E.Coli
Advances in Experimental Medicine and Biology, pp. 5–16 2016

Miller, H., Wollman, A.J.M., and Leake, M.C.
Designing a single-molecule biophysics tool for characterising DNA damage for techniques that kill infectious pathogens through DNA damage effects.
Advances in Experimental Medicine and Biology, pp. 115–127 2016

Adam J M Wollman,* and Helen Miller,* Simon Foster, Mark C Leake
An automated image analysis framework for segmentation and division plane detection of single live Staphylococcus aureus cells which can operate at millisecond sampling time scales using bespoke Slimfield microscopy
Physical Biology, 13, 55002 2016

2015

Adam J M Wollman, Helen Miller, Zhaokun Zhou, Mark C Leake
Probing DNA interactions with proteins using a single-molecule toolbox: inside the cell, in a test tube, and in a computer
Biochemical Society Transactions, 43(2), 139 145. 2015

Helen Miller, Zhaokun Zhou, Adam J M Wollman, Mark C Leake
Superresolution imaging of single DNA molecules using stochastic photoblinking of minor groove and intercalating dyes
Methods,2015

Adam J M Wollman, Richard Nudd, Erik Hedlund, Mark C Leake
From Animalculum to Single Molecules: 300 years of the light microscope
Open Biology, 5(4), 150019–150019 2015

Zhaokun Zhou, Helen Miller, Adam J M Wollman, Mark C Leake
Developing a New Biophysical Tool to Combine Magneto-Optical Tweezers with Super-Resolution Fluorescence Microscopy
Photonics. Multidisciplinary Digital Publishing Institute.  2015

Adam J M Wollman, and Mark C Leake
Millisecond single-molecule localization microscopy combined with convolution analysis and automated image segmentation to determine protein concentrations in complexly structured, functional cells, one cell at a time
Faraday Discussions, 184, 401–424 2015

2014

Adam J M Wollman, Carlos Sanchez-Cano, Helen M J Carstairs, Robert A Cross, Andrew J Turberfield
Transport and self-organization across scales powered by motor proteins and programmed by DNA
Nature Nanotechnology, 9(1),447. 2014

Pre-prints

Helen Miller, Jason Cosgrove, Adam J. M. Wollman, Peter J. O’Toole, Mark Coles,  Mark C. Leake. Ultra-fast super-resolution imaging of biomolecular mobility in tissues. BioRxiv – https://doi.org/10.1101/179747

Karita Haapasalo* and Adam Wollman*, Carla de Haas, Kok van Kessel, Jos van Strijp, Mark C Leake. Staphylococcus aureus toxin LukSF dissociates from its membrane receptor target to enable renewed ligand sequestration. BioRxiv – https://doi.org/10.1101/251645