Publications

2020

Adam JM Wollman, Katarina Muchová, Zuzannah Chromiková, Anthony Wilkinson, Imrich Barák, Mark Leake, Single-Molecule Optical Microscopy of Protein Dynamics and Computational Analysis of Images to Determine Cell Structure Development in Differentiating Bacillus Subtilis. Computational and Structural Biotechnology Journal 2020. https://doi.org/10.1016/j.csbj.2020.06.005.

Helen Miller, Sonia Contera, Adam JM Wollman, Adam Hirst, Katherine Dunn, Sandra Schröter, Deborah O’Connell, Mark Leake, Biophysical Characterisation of DNA Origami Nanostructures Reveals Inaccessibility to Intercalation Binding Sites. Nanotechnology 2020, 31 (23), 235605. https://doi.org/10.1088/1361-6528/ab7a2b.

2019

Aisha Syeda* and  Adam J M Wollman,*  Alex Hargreaves, Jamieson Howard, Jan-Gert Brüning,  Peter McGlynn, Mark Leake,  Single-molecule live cell imaging of Rep reveals the dynamic interplay between an accessory replicative helicase and the replisome. Nucleic Acids Res (2019) doi:10.1093/nar/gkz298.

Adam J M Wollman, Erik Hedlund, Sviatlana Shashkova, & Mark Leake,  Towards mapping the 3D genome through high speed single-molecule tracking of functional transcription factors in single living cells. Methods (2019) doi:10.1016/j.ymeth.2019.06.021.

Yaqi Sun* and Adam J M Wollman,* Fang Huang, Mark Leake, Luning Lui
Single-organelle quantification reveals the stoichiometric and structural variability of carboxysomes dependent on the environment
Plant Cell .  https://doi.org/10.1105/tpc.18.00787

2018

Karita Haapasalo* and Adam Wollman*, Carla de Haas, Kok van Kessel, Jos van Strijp, Mark C Leake
Staphylococcus aureus toxin LukSF dissociates from its membrane receptor target to enable renewed ligand sequestration
FASEB – doi: 10.1096/fj.201801910R 2018

Mathew Stracy* and Adam J.M. Wollman*, Elzbieta Kaja, Jacek Gapinski, Ji-Eun Lee, Victoria A. Leek, Shannon J. McKie, Lesley A. Mitchenall, Anthony Maxwell, David J.Sherratt, Mark C. Leake, Pawel Zawadzki
Single-molecule imaging of DNA gyrase activity in living Escherichia coli
Nucleic Acids Research, doi: 10.1093/nar/gky1143 2018

Helen Miller,* Jason Cosgrove,* Adam J. M. Wollman, Peter J. O’Toole, Mark Coles, Mark C. Leake
High-speed single-molecule tracking of CXCL13 in the B-Follicle
Frontiers of Immunology, 9, p1073 2018

Sviatlana Shashkova* and Adam J M Wollman,* Stefan Hohmann, Mark C Leake. Characterising Maturation of GFP and mCherry of Genomically Integrated Fusions in Saccharomyces cerevisiae. Bio-protocol, 8, 2

Victoria A. Lund, Katarzyna Wacnik, Robert D. Turner, Bryony E. Cotterell, Samuel J. Fenn, Christa G. Walther, Fabien Grein, Adam J. M. Wollman, Mark C. Leake, Nicolas Olivier, Ashley Cadby, Stephané Mesnage, Simon Jones, Simon Foster                    Molecular coordination mechanism of Staphylococcus aureus cell wall construction eLife, 7:e32057

2017

Beattie, T.R., Kapadia, N., Nicolas, E., Uphoff, S., Wollman, A.J.M., Leake, M.C. & Reyes-Lamothe, R
Synthesis by the bacterial replisome occurs during frequent exchange of the DNA polymerase
eLife, ;6:e21763 2017

Shashkova, S.* and Wollman, A.J.M.,* Leake, M.C., Hohmann, S
The yeast Mig1 transcriptional repressor is dephosphorylated by glucose-dependent and independent mechanisms
FEMS Microbiology Letters, 364,14, 2017

Wollman, A.J.M.* and Shashkova, S.,* Hedlund, E.G., Friemann, R., Hohmann, S., Leake, M.C.
Transcription factor clusters regulate genes in eukaryotic cells
eLife, 6:e274512017

Dunn, K.E., Leake, M.C., Wollman, A.J.M., Trefzer, M.A., Johnson, S.  and Tyrrell, A.M.
An experimental study of a synthetic autonomous rotary DNA nanomotor driven by strand displacement.
Open Science, 4 (3), 160767 2017

Helen Miller, Zhaokun Zhou, Jack Shepherd, Adam J M Wollman, Mark C Leake
Single-molecule techniques in biophysics: a review of the progress in methods and applications
In Press, Reports on progress in physics, 2017

2016

Adam J M Wollman and Mark C Leake
Single Molecule Narrowfield Microscopy of Protein-DNA binding dynamics in glucose signal transduction of live yeast cells
Chromosom. Archit. Methods Protoc., 5–15.2016

Adam J M Wollman, Aisha Syeda, Peter McGlynn, Mark C Leake
Single-molecule observation of DNA replication repair pathways in E.Coli
Advances in Experimental Medicine and Biology, pp. 5–16 2016

Miller, H., Wollman, A.J.M., and Leake, M.C.
Designing a single-molecule biophysics tool for characterising DNA damage for techniques that kill infectious pathogens through DNA damage effects.
Advances in Experimental Medicine and Biology, pp. 115–127 2016

Adam J M Wollman,* and Helen Miller,* Simon Foster, Mark C Leake
An automated image analysis framework for segmentation and division plane detection of single live Staphylococcus aureus cells which can operate at millisecond sampling time scales using bespoke Slimfield microscopy
Physical Biology, 13, 55002 2016

2015

Adam J M Wollman, Helen Miller, Zhaokun Zhou, Mark C Leake
Probing DNA interactions with proteins using a single-molecule toolbox: inside the cell, in a test tube, and in a computer
Biochemical Society Transactions, 43(2), 139 145. 2015

Helen Miller, Zhaokun Zhou, Adam J M Wollman, Mark C Leake
Superresolution imaging of single DNA molecules using stochastic photoblinking of minor groove and intercalating dyes
Methods,2015

Adam J M Wollman, Richard Nudd, Erik Hedlund, Mark C Leake
From Animalculum to Single Molecules: 300 years of the light microscope
Open Biology, 5(4), 150019–150019 2015

Zhaokun Zhou, Helen Miller, Adam J M Wollman, Mark C Leake
Developing a New Biophysical Tool to Combine Magneto-Optical Tweezers with Super-Resolution Fluorescence Microscopy
Photonics. Multidisciplinary Digital Publishing Institute.  2015

Adam J M Wollman, and Mark C Leake
Millisecond single-molecule localization microscopy combined with convolution analysis and automated image segmentation to determine protein concentrations in complexly structured, functional cells, one cell at a time
Faraday Discussions, 184, 401–424 2015

2014

Adam J M Wollman, Carlos Sanchez-Cano, Helen M J Carstairs, Robert A Cross, Andrew J Turberfield
Transport and self-organization across scales powered by motor proteins and programmed by DNA
Nature Nanotechnology, 9(1),447. 2014